Assignment of syndecan 2 (SDC2)gene to cattle chromosome band 14q22 and thymus high mobility group box protein TOX (TOX)(2) gene to cattle chromosome band 14q17-->q18 by in situ hybridization.

Syndecan 2 gene codes for a transmembrane heparan sulfate proteoglycan protein that has an unclear function at the cell surface. The tumorigenic activity of SDC2 in colon carcinoma cell behavior and its influence on the regulation of adhesion and proliferation in colon carcinoma cell has been described (Park et al., 2002). TOX represents a thymus high mobility group box protein that is involved in the regulation of thymocyte selection (Wilkinson et al., 2002). Human SDC2 (alternate HSPG) was assigned on chromosome band HSA8q22→q23 (Marynen et al., 1989) and human TOX (alternate KIAA0808) was mapped on position 325.53 cR3000 in the Genebridge 4 RH-Map (Gyapay et al., 1996), which corresponds to chromosome region HSA8q11.23. We report here the localization of SDC2 and TOX on cattle chromosome BTA14 by fluorescence in situ hybridization. Materials and methods